Direct observation of DNA dynamics in semidilute solutions in extensional flow
نویسندگان
چکیده
منابع مشابه
Direct observation of single molecule mobility in semidilute polymer solutions.
We determine the mobility of dye-labeled polystyrene molecules in solution by fluorescence correlation spectroscopy (FCS) over a wide range of concentrations and molecular weights (ranging from 3.9 x 10{3} to 1550 x 10{3} g/mol ). In order to obtain absolute values of the diffusion coefficient, which can be compared to diffusion coefficients determined by other methods, the size of the focal vo...
متن کاملElectro-osmotic flow of semidilute polyelectrolyte solutions.
We investigate electro-osmosis in aqueous solutions of polyelectrolytes using mean-field equations. A solution of positively charged polyelectrolytes is confined between two negatively charged planar surfaces, and an electric field is applied parallel to the surfaces. When electrostatic attraction between the polymer and the surface is strong, the polymers adhere to the surface, forming a highl...
متن کاملObservation of polymer conformation hysteresis in extensional flow.
Highly extensible Escherichia coli DNA molecules in planar extensional flow were visualized in dilute solution by fluorescence microscopy. For a narrow range of flow strengths, the molecules were found in either a coiled or highly extended conformation, depending on the deformation history of the polymer. This conformation hysteresis persists for many polymer relaxation times and is due to conf...
متن کاملExtensional flow of blood analog solutions in microfluidic devices.
In this study, we show the importance of extensional rheology, in addition to the shear rheology, in the choice of blood analog solutions intended to be used in vitro for mimicking the microcirculatory system. For this purpose, we compare the flow of a Newtonian fluid and two well-established viscoelastic blood analog polymer solutions through microfluidic channels containing both hyperbolic an...
متن کاملMobility of Nanoparticles in Semidilute Polyelectrolyte Solutions
We measure the mobility of nanoparticles at low concentrations in non-Newtonian semidilute aqueous solutions of high-molecular-weight polyelectrolyte polymers. Using optical microscopy and particle tracking algorithms, we image and track hydrophilic polystyrene nanoparticles of diameter 400 nm moving in aqueous solutions of partially hydrolyzed polyacrylamide of molecular weight 8 000 000 Da an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Rheology
سال: 2017
ISSN: 0148-6055,1520-8516
DOI: 10.1122/1.4972236